Is the viability analysis of the cell counter accurate? The results from the Trypan Blue experiment will tell you.

Release time:

2025-01-08

Source:

Newtonian Optics

Newton optical cell counter is accurate and reliable for counting live cell viability.

Newton Optics

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Trypan Blue viability experiment

01Introduction

In the exploration journey of cell biology, we often face a key question: how to accurately measure cell viability? This not only relates to the accuracy of experimental data but also directly affects the reliability of research conclusions. Cell counters, as effective assistants in modern biological laboratories, have naturally become the focus of researchers regarding the accuracy of their viability analysis functions.

Today, we will focus on a core issue——The accuracy of viability counting of cell counters under the Trypan Blue mode. Through this staining method, we can observe that the cell membrane of live cells can exclude Trypan Blue, maintaining its original color; while dead cells, due to compromised membrane integrity, have increased permeability, allowing Trypan Blue to penetrate the cell interior and stain it blue. Therefore, it can clearly distinguish between live and dead cells. However, when this technology is combined with modern cell counters, can the results withstand scientific scrutiny?

We conducted an in-depth exploration of this issue through a carefully designed Trypan Blue viability experiment.

02Experimental Purpose

By preparing several samples with known viability(Theoretical viability)ofHEK293cell samples, andperforming Trypan Blue staining, andand using a cell counter to measure viability, we verify the accuracy of the cell counter's cell viability analysis.

03Experimental plan

1, after reviving the cellsHEK293use, andPBSto resuspend the cellsThe cell condition is good, andObserve the cells under a microscope, andTheir viability is about, and99%;, heat in a water bath to inactivate some cells

2By mixing live and dead cells in proportion, andwe obtain theoretical viabilities of, and90%,ofCell170%45% Cell3;ofCell270%Using Newton OpticsofVenus

3. fully automatic high-throughput cell counter to analyze the three different viability samples three times eachand take the average.The cell counter used, andResults display

Left image: Trypan Blue counting results

04Right image: Trypan Blue counting marked results

Theoretical viability and instrument measured viability Conclusion

The counting results of the Newton Optics cell counter are close to the theoretical values for high, medium-high, and medium viability.

05The Trypan Blue viability counting results of the Newton Optics cell counter are accurate.

END, andGuangzhou Newton Optics Research Institute Co., Ltd.

Located in Tianhe, Guangzhou, it is an innovative enterprise focused on the application of intelligent imaging technology in the life sciences field. The company focuses on the field of cell analysis and has established a complete R&D, production, and sales system.

Newton Optics adheres to the value principles of integrity, responsibility, and excellence, aiming to bring innovative and extreme scientific tools to customers in the global life sciences field.Service hotline:

020-38808574