The application of the Newton Optical Jupita Fluorescent Cell Analyzer in the user's Haoyang Biological Cell Library Project
Release time:
2024-12-13
Source:
Newtonian Optics
Reprinted from user Haoyang Biology: Jupita - A fast and accurate Newtonian optical fluorescence cell analyzer
Newton Optics
Intelligent imaging, all in one step!
Fast and accurate counting——This is our most basic and highest requirement for counting instruments.
Human immune cells,IPSThe development of the cell bank project
01Project Introduction
By collecting peripheral blood, isolating immune cells, and storing them in a professional cell storage facility that meets below196°Cenvironment, using ultra-low temperature technology to preserve their activity for a long time. When needed in the future, it provides health protection.etc.
Tianjin Haoyang provides all reagents, consumables, and technical guidance services for the establishment and cryopreservation of human immune cell banks:
02Excellent performance of the fluorescent cell counter in the project
This project involves a large amount of cell counting work, and since the counted cells are from peripheral blood, the components in the samples are complex, especially with possible contamination from red blood cells, which are similar in size toPBMC, this makes traditional counting methods unable to meet technical requirements.
PBMCAfter mixing the sample with fluorescent dye, the nuclei of individual nucleated cells are stained, while red blood cells cannot be stained due to being anucleate, making it perfectly suitable for the counting work of this project.
The cell counter used in this project comes fromNewton OpticsJupitaFluorescent Cell Analyzer.The following is the instrument interface, which cansimultaneously display the counting results of multiple samples, including quantity, viability, average cell diameter, aggregation rateand so on.
The following shows the difference between bright field counting and fluorescent counting of samples. It can be seen that due to the presence of platelets and red blood cells in the bright field, the view is very messy, and the counting is inaccurate. Under fluorescence, nucleated cells are clearly displayed, and the counting is accurate and clear.
The following is a comparison of counting data between the fluorescent counter and flow cytometer, which shows that the counting results are not significantly different. (Due to sampling, mixing, instrument, and other errors, complete elimination is not possible)
03 IPSSubsequent applications of the cell bank project
After collecting peripheral blood, prepareIPScells.
IPSCell quality standards:
1.DetectionhiPSCs' pluripotency markersOct4,Sox2,TRA-1-81,NanogStem cell marker genes are stably expressed in more than90%of the cells.
2.Karyotype testing is qualified, excluding abnormal chromosome recombination.
3.Provide short tandem repeat(short tandem repeat,STR)detection report, establishing a uniqueDNAidentity for each sample.
4.Provide detection reports, with no fungal, bacterial, or mycoplasma contamination.
The above text and images are reproduced from"Haoyang Biology"
Product Overview
END
Guangzhou Newton Optical Research Institute Co., Ltd.is located in Tianhe, Guangzhou, and is an innovative enterprise focused on the application of intelligent imaging technology in the life sciences. The company focuses on the field of cell analysis and has established a complete R&D, production, and sales system.
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