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Tuger empowers Hubei University of Chinese Medicine: PDX model quality control enters the era of precision

Release time:

2025-11-06

Source:

Newtonian Optics

Newtonian Optics

Smart imaging—done in one step!

Hubei University of Traditional Chinese Medicine

School Introduction

Hubei University of Traditional Chinese Medicine (Hubei University of Chinese Medicine, abbreviated as “Hu Zhongyi” or “HBUCM”) ) is a university jointly established by the People's Government of Hubei Province and the State Administration of Traditional Chinese Medicine, and it also ranks among Hubei Province’s "Universities for First-Class Discipline Development within China." Universities participating in the "National Project for Enhancing Basic Capabilities of Universities in Central and Western China." Located in Wuhan, Hubei Province, the university upholds the school motto of "Diligence, Pragmatism, Exploration, and Innovation," dedicating itself to cultivating talents in traditional Chinese medicine, advancing scientific research, providing societal services, and fostering innovative cultural heritage.

Hubei University of Traditional Chinese Medicine

Experimental Background

The PDX mouse model (patient-derived tumor xenograft model) is a crucial tool in modern cancer research. It involves directly transplanting a patient’s tumor tissue into immunodeficient mice, creating a preclinical research platform that closely mimics the original tumor’s heterogeneity, microenvironment, and molecular characteristics.

Building a PDX model is a systematic and meticulous process. First, fresh tumor tissue samples from patients—typically obtained through surgery or biopsy—must be collected and promptly preserved in a low-temperature culture medium to maintain cellular viability. After removing necrotic tissue and thoroughly washing the samples, they are processed into small pieces measuring approximately 1–2 mm³ or converted into single-cell suspensions. These prepared tumor samples are then transplanted into immunodeficient mice. The choice of mouse strain is critical; commonly used models include nude mice, NOD/SCID mice, and even more severely immunodeficient NSG mice. Notably, the higher the degree of immunodeficiency in the mouse, the greater the likelihood of successful engraftment, as this effectively prevents the host mouse immune system from rejecting the human tumor tissue.

Compared to traditional cell line-derived xenograft (CDX) models, the unique value of PDX models lies in their ability to preserve, to the greatest extent possible, the heterogeneity of the parental tumor as well as its intricate tumor microenvironment. This means that PDX models not only retain the diverse subpopulations of cancer cells within the tumor but also maintain the original stromal cells, vascular structures, and other components—thereby enabling a more accurate simulation of the tumor’s true state and biological behavior as it exists in the human body.

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Highly efficient and precise cell analysis solutions

In the process of constructing PDX (patient-derived tumor xenograft) mouse models, the cell counter plays a critical role as the core component for quality control. Tuger precisely measures the concentration and viability of tumor cell suspensions. (Usually using staining methods such as Trypan Blue to distinguish between live and dead cells), ensure that only cells capable of survival are transplanted into immunodeficient mice each time. Active compliance, precise quantity This cellular population not only directly determines the success rate of transplantation and the efficiency of model construction, but also significantly minimizes human error through its highly automated and standardized analysis—such as completing cell counting within 30 seconds and automatically exporting data. This ensures a reliable, reproducible, quantitative foundation for subsequent experiments, thereby safeguarding the accuracy and reliability of PDX models in accurately simulating the heterogeneity of original tumors, evaluating drug responses, and supporting personalized medicine research.

Newton Optics Tuger Fully Automatic Fluorescence Cell Analyzer

Tuger On-site Installation and Adjustment

Newton Optics is honored to contribute to scientific research at Hubei University of Chinese Medicine. Newton Optics is committed to providing researchers with higher-quality, more convenient experimental solutions.

Product Overview

END

Guangzhou Newton Optics Research Institute Co., Ltd. Located in Tianhe, Guangzhou, it is an innovative enterprise specializing in the application of intelligent imaging technology in the life sciences. The company focuses on the field of cell analysis and has already established a comprehensive R&D, production, and sales system.

Newton Optics upholds the values of integrity, responsibility, and excellence, and is committed to delivering cutting-edge, innovative scientific tools to customers in the global life sciences field.

Service Hotline: 400-9613721

Customer service phone number: 15322243697 (WeChat)

Official website: www.newtonoptic.com

R&D Center: No. 38 Gaopu Road, Building 1, North Wing, 3rd Floor, Tianhe District, Guangzhou City

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